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    以本教研室多年来厚实的科学研究积累为基础,突出汕头大学医学院特色,把实验教学提升到一个更高的层次予以重新设计,对实验教学内容实施系列改造,重视把新的实验技术手段方法及时引入实验教学,强化实验的综合性与设计性特质,深化实验教学内涵,把汕头大学医学院生物化学与分子生物学学科的科研成果优势转化为促进深化实验教学的强有力因素,成效十分显著。

              1998年以来,本课程团队,以二级学科生物化学与分子生物学硕士点/博士点,以及一级学科基础医学博士后流动站,一级学科生物学博士后流动站为依托,在科学研究上成果丰富,累计获得国家自然科学基金系列,重点项目3项,面上项目12项,青年基金6,基于此,累计发表SCI研究论文150余篇,获得中国发明专6项。代表性研究论文的目录见下。

代表性研究论文(#共同第一作者,*通讯作者或共同通讯作者):

1. Fang WK, Xu LY*, Lu XF, Liao LD, Cai WJ, Shen ZY, Li EM*. A novel alternative spliced variant of neutrophil gelatinase-associated lipocalin receptor in esophageal carcinoma cells. Biochem J. 2007;403(2):297-303.

2. Cui L, Xu LY, Shen ZY, Tao Q, Gao SY, Lv Z, Du ZP, Fang WK, Li EM*. NGALR is overexpressed and regulated by hypomethylation in esophageal squamous cell carcinoma. Clin Cancer Res. 2008;14(23):7674-7681.

3. Xie JJ, Xu LY*, Xie YM, Zhang HH, Cai WJ, Zhou F, Shen ZY, Li EM*. Roles of ezrin in the growth and invasiveness of esophageal squamous carcinoma cells. Int J Cancer. 2009;124(11):2549-2558.

4. Gao SY, Li EM*, Cui L, Lu XF, Meng LY, Yuan HM, Xie JJ, Du ZP, Pang JX, Xu LY*. Sp1 and AP-1 regulate expression of the human gene VIL2 in esophageal carcinoma cells. J Biol Chem. 2009;284(12):7995-8004.

5. Xie JJ, Xu LY*, Wu JY, Shen ZY, Zhao Q, Du ZP, Lv Z, Gu W, Pan F, Xu XE, Xie D*, Li EM*. Involvement of CCN1 and CCN2 in the fascin-mediated proliferation and invasiveness of esophageal squamous cell carcinomas cells. Am J Pathol. 2010;176(2):939-951.

6. Lu XF, Li EM*, Du ZP, Xie JJ, Guo ZY, Gao SY, Liao LD, Shen ZY, Xie D*, Xu LY*. Specificity protein 1 regulates fascin expression in esophageal squamous cell carcinoma as the result of the epidermal growth factor/extracellular signal-regulated kinase signaling pathway activation. Cell Mol Life Sci. 2010;67(19):3313-3329.

7. Fang WK, Gu W, Li EM*, Wu ZY, Shen ZY, Shen JH, Wu JY, Pan F, Lv Z, Xu XE, Huang Q, Xu LY*. Reduced membranous and ectopic cytoplasmic expression of DSC2 in esophageal squamous cell carcinoma: an independent prognostic factor. Hum Pathol. 2010;41 (10):1456-1465.

8. Du ZP, Lv Z, Wu BL, Wu ZY, Shen JH, Wu JY, Xu XE, Huang Q, Shen J, Chen HB, Li EM*, Xu LY*. Neutrophil gelatinase-associated lipocalin and its receptor: independent prognostic factors of oesophageal squamous cell carcinoma. J Clin Pathol. 2011;64(1):69-74.

9. Du ZP, Yuan HM, Wu BL, Chang JX, Lv Z, Shen J, Wu JY, Chen HB, Li EM*, Xu LY*. Neutrophil gelatinase-associated lipocalin in gastric carcinoma cells and its induction by TPA are controlled by C/EBPβ. Biochem Cell Biol. 2011;89(3):314-324.

10. Li TY, Xu LY*, Wu ZY, Liao LD, Shen JH, Xu XE, Du ZP, Zhao Q, Li EM*. Reduced nuclear and ectopic cytoplasmic expression of lysyl oxidase-like 2 is associated with lymph node metastasis and poor prognosis in esophageal squamous cell carcinoma. Hum Pathol. 2012;43(7):1068-1076.

11. Fang WK, Liao LD, Li LY, Xie YM, Xu XE, Zhao WJ, Wu JY, Zhu MX, Wu ZY, Du ZP, Wu BL, Xie D, Guo MZ, Xu LY*, Li EM*. Down-regulated desmocollin-2 promotes cell aggressiveness through redistributing adherens junctions and activating beta-catenin signalling in oesophageal squamous cell carcinoma. J Pathol. 2013;231(2):257-270.

12.   Hou J, Liao LD, Xie YM, Zeng FM, Ji X, Chen B, Li LY, Zhu MX, Yang CX,Qing-Zhao, Chen T, Xu XE, Shen J, Guo MZ*, Li EM*, Xu LY*. DACT2 is a candidate tumor suppressor and prognostic marker in esophageal squamous cell carcinoma. Cancer Prev Res (Phila). 2013;6(8):791-800.

13. Xu YW, Peng YH, Chen B, Wu ZY, Wu JY, Shen JH, Zheng CP, Wang SH, Guo HP, Li EM*, Xu LY*. Autoantibodies as potential biomarkers for the early detection of esophageal squamous cell carcinoma. Am J Gastroenterol. 2014;109(1):36-45.

14. Zhang HF, Zhang K, Liao LD, Li LY, Du ZP, Wu BL, Wu JY, Xu XE, Zeng FM, Chen B, Cao HH, Zhu MX, Dai LH, Long L, Wu ZY, Lai R, Xu LY*, Li EM*. MiR-200b suppresses invasiveness and modulates the cytoskeletal and adhesive machinery in esophageal squamous cell carcinoma cells via targeting Kindlin-2. Carcinogenesis. 2014;35(2):292-301.

15. Song Y#, Li L#, Ou Y#, Gao Z#, Li E#, Li X#, Zhang W, Wang J, Xu L, Zhou Y, Ma X, Liu L, Zhao Z, Huang X, Fan J, Dong L, Chen G, Ma L, Yang J, Chen L, He M, Li M, Zhuang X, Huang K, Qiu K, Yin G, Guo G, Feng Q, Chen P, Wu Z, Wu J, Ma L, Zhao J, Luo L, Fu M, Xu B, Chen B, Li Y, Tong T, Wang M, Liu Z, Lin D, Zhang X, Yang H,Wang J, Zhan Q*. Identification of genomic alterations in oesophageal squamous cell cancer. Nature. 2014;509(7498):91-95.

16. Xie JJ, Xie YM, Chen B, Pan F, Guo JC, Zhao Q, Shen JH, Wu ZY, Wu JY, Xu LY*, Li EM*. ATF3 functions as a novel tumor suppressor with prognostic significance in esophageal squamous cell carcinoma. Oncotarget. 2014;5(18):8569-8582.

17. Wu B, Li C, Du Z, Yao Q, Wu J, Feng L, Zhang P, Li S, Xu L*, Li E*. Network based analyses of gene expression profile of LCN2 overexpression in esophageal squamous cell carcinoma. Sci Rep. 2014;4:5403.

18. Tan H, Zhang H, Xie J, Chen B, Wen C, Guo X, Zhao Q, Wu Z, Shen J, Wu J, Xu X, Li E*, Xu L*, Wang X*. A novel staging model to classify oesophageal squamous cell carcinoma patients inChina. Br J Cancer. 2014;110(8):2109-2115.

19. Sun LL, Wu JY, Wu ZY, Shen JH, Xu XE, Chen B, Wang SH, Li EM*, Xu LY*. A three-gene signature and clinical outcome in esophageal squamous cell carcinoma. Int J Cancer. 2015;136(6):E569-577.

20. Cao HH, Zhang SY, Shen JH, Wu ZY, Wu JY, Wang SH, Li EM*, Xu LY*. A three-protein signature and clinical outcome in esophageal squamous cell carcinoma. Oncotarget. 2015;6(7):5435-5448.

21. Zhang K*, Li L, Zhu M, Wang G, Xie J, Zhao Y, Fan E, Xu L*, Li E*. Comparativeanalysis of histone H3 and H4 post-translational modifications of esophagealsquamous cell carcinoma with different invasive capabilities. J Proteomics. 2015;112:180-189.

22. Huang GW, Liao LD, Li EM*, Xu LY*. siRNA induces gelsolin gene transcriptionactivation in human e sophageal cancer cell. Sci Rep. 2015 Jan 20;5:7901.

23. Li LY#, Zhang K#, Jiang H#, Xie YM, Liao LD, Chen B, Du ZP, Zhang PX, Chen H,Huang W, Jia W, Cao HH, Zheng W*, Li EM*, Xu LY*. Quantitative proteomics revealsthe downregulation of GRB2 as a prominent node of F806-targeted cellproliferation network. J Proteomics. 2015;117:145-55.

24. Li LY#, Jiang H#, Xie YM, Liao LD, Cao HH, Xu XE, Chen B, Zeng FM, Zhang YL, Du ZP, Chen H, Huang W, Jia W, Zheng W, Xie JJ, Li EM*, Xu LY*. Macrolide analog F806 suppresses esophageal squamous cell carcinoma (ESCC) by blocking β1 integrinactivation. Oncotarget. 2015;6(18):15940-15952.

25. Du ZP, Wu BL*, Wu X, Lin XH, Qiu XY, Zhan XF, Wang SH, Shen JH, Zheng CP, WuZY, Xu LY*, Wang D, Li EM. A systematic analysis of human lipocalin family and itsexpression in esophageal carcinoma. Sci Rep. 2015 Jul 1;5:12010.

26. Peng YH#, Xu YW#, Huang LS, Zhai TT, Dai LH, Qiu SQ, Yang YS, Chen WZ, Zhang LQ,Li EM*, Xu LY*. Autoantibody signatures combined with Epstein-Barr virus capsidantigen-IgA as a biomarker panel for the detection of nasopharyngeal carcinoma. Cancer Prev Res (Phila). 2015;8(8):729-736.

27. Du ZP, Wu BL, Xie YM, Zhang YL, Liao LD, Zhou F, Xie JJ, Zeng FM, Xu XE, Fang WK, Li EM*, Xu LY*. Lipocalin 2 promotes the migration and invasion of esophagealsquamous cell carcinoma cells through a novel positive feedback loop. Biochim Biophys Acta. 2015;1853(10 Pt A):2240-2250.

28. Li G, He S, Chang L, Lu H, Zhang H, Zhang H, Chiu J. GADD45α and annexin A1 are involved in the apoptosis of HL-60 induced by resveratrol. Phytomedicine. 2011;18(8-9):704-709.

29. Li G, Lee LS#, Li M, Tsao SW, Chiu JF. Molecular Changes during Arsenic-Induced Cell Transformation. J Cell Physiol. 2011;226(12):3225-3232.

30. Jiao RQ, Li G, Chiu JF. Comparative proteomic analysis of differentiation of mouse F9 embryonic carcinoma cells induced by retinoic acid. J Cell Biochem. 2012;113(6):1811-1819.

31. Tang Q, Li G#, Wei X, Zhang J, Chiu JF, Hasenmayer D, Zhang D, Zhang H. Resveratrol-induced apoptosis is enhanced by inhibition of autophagy in esophageal squamous cell carcinoma. Cancer Lett. 2013;336(2):325-337.

32. Jun Zhang, JenFu Chiu, Hongwei Zhang, Tingting Qi, Qishan Tang, Ke Ma, Hong Lu, Guanwu Li*, Autophagic cell death induced by resveratrol depends on the Ca2+/AMPK/mTOR pathway in A549 cells. Biochemical Pharmacology. 2013;86(2):317-328.

33. Jun Zhang, Ke Ma, Tingting Qi, Xiaoning Wei, Qing Zhang, Guanwu Li* and Jen-Fu Chiu*. P62 regulates resveratrol-mediated Fas/Cav-1 complex formation and transition from autophagy to apoptosis. Oncotarget. 2015;6(2):789-801.